--- title: Optimizing HTS Microtiter Plate Selection with our Product Finder description: We discuss microtiter plate selection and recommended practices in high throughput screening and quantitative biology. image: https://focus.gbo.com/hubfs/HTS%20Kampagne/HTS%20Microplate/CP01_Microplate%20Selection_Awareness_01_840x540px.jpg --- [![GBO](https://focus.gbo.com/hubfs/raw_assets/public/Greiner_Bio_One_April2023/images/logo.svg "GBO")](https://focus.gbo.com/blog/hts) - [HTS Solutions - Take your Next Step in High-Throughput Screening](https://focus.gbo.com/blog/hts) - [Contact our expert](https://focus.gbo.com/contact-hts-expert) ![](https://focus.gbo.com/hubfs/HTS%20Kampagne/HTS%20Microplate/CP01_Microplate%20Selection_Awareness_01_840x540px.jpg) [Back to Blog](https://focus.gbo.com/blog/hts) # Optimizing HTS Microtiter Plate Selection with our Product Finder This article introduces the Greiner Bio-One product selector to help you select the right microtiter plate for your high-throughput screening campaign. We discuss microtiter plate selection and recommended practices in high-throughput screening and quantitative biology. ### Go directly to the Product Finder: [![Product Finder](https://hubspot-no-cache-eu1-prod.s3.amazonaws.com/cta/default/25665555/13141798-b490-4ac2-8157-ea297b137d55.png)](https://hubspot-cta-redirect-eu1-prod.s3.amazonaws.com/cta/redirect/25665555/13141798-b490-4ac2-8157-ea297b137d55) ## Microtiter plates by application: Tell me your technology and I’ll tell you what you need The surface properties of screening microtiter plates must be adapted to the research question at hand. For example, protein adsorption on microtiter plates increases in the following order:   --- non-binding polystyrene  >  polypropylene  >  untreated polystyrene  >  high-binding polystyrene\[1\] --- ## Surface and bottom considerations for biochemical screening ### Polystyrene (untreated) Polystyrene microplates without surface treatment are characterized by low binding of biomolecules and are often used for biochemical screening applications. ### Polypropylene (untreated) Polypropylene microtiter plates are used for storage applications and biochemical assays. They show lower biomolecule adsorption than polystyrene microtiter plates. ### Polystyrene (non-binding) Non-binding polystyrene microtiter plates are particularly suitable for sensitive biochemical assays. Since molecules such as proteins, peptides and nucleic acids are bound to a very low extent, these plates achieve high assay sensitivity and improved signal-to-noise ratio. ### Polystyrene (medium-binding) Medium-binding polystyrene microtiter plates are suitable for heterogeneous / immunological assays such as ELISA, FIA and LIA. The incoming raw material used for ELISA microplates is routinely monitored for identity and immunological quality to ensure a stable coefficient of variation (CV) from batch to batch. ### Polystyrene (high-binding) High-binding polystyrene microtiter plates are also suitable for heterogeneous / immunological assays. In comparison to medium-binding plates those plates possess a higher binding capacity. ### Polystyrene (streptavidin-coated) Streptavidin is a useful tool for many molecular biology applications. The very tight bond between streptavidin and biotin can be utilized to attach biotin-labeled proteins, nucleic acids, or other compounds to the streptavidin-coated microplate. ![CP02_Microplate Selection_Decision_Base Materials](https://focus.gbo.com/hs-fs/hubfs/HTS%20Kampagne/HTS%20Microplate/CP02_Microplate%20Selection_Decision_Base%20Materials.png?width=497&height=200&name=CP02_Microplate%20Selection_Decision_Base%20Materials.png)   Due to the high clarity of polystyrene, plates made of this material are ideal for colorimetric measurements in the visible range. It should be noted that the transmittance of polystyrene products decreases sharply at approximately 400 nm. When a lower wavelength range is needed, Greiner Bio-One offers [thin transparent film bottoms](https://shop.gbo.com/en/row/products/bioscience/microplates/96-well-microplates/96-well-microplates-uclear/) in black or white framed µClear® plates. These enable detection capabilities down to 340 nm and are also ideally suited for standard microscopic applications. For measurements in the lower UV range, which is used, for example, for DNA or protein concentrations, there are [UV-Star® microplates made of cycloolefin](https://shop.gbo.com/en/row/products/bioscience/microplates/uv-star-microplates/) with transmission down to 230 nm.   #### ![CP02_Microplate Selection_Decision_Wavelength](https://focus.gbo.com/hs-fs/hubfs/HTS%20Kampagne/HTS%20Microplate/CP02_Microplate%20Selection_Decision_Wavelength.png?width=575&height=283&name=CP02_Microplate%20Selection_Decision_Wavelength.png) ## Greiner Bio-One product finder The success of high-throughput screening can be significantly influenced by the careful selection of microtiter plates and the application of best practices. One must keep in mind that microtiter plate surfaces are not uniform and can significantly affect the results of the assay. Key parameters such as the number of wells, microtiter plate color, well volume, well shape, and microtiter plate surface coating are of particular importance. Experiments including incubation and dosage of substances and reagents should also be carefully optimized. Choose the right high-throughput screening microtiter plate for your application with our product finder:   [![Product Finder](https://hubspot-no-cache-eu1-prod.s3.amazonaws.com/cta/default/25665555/13141798-b490-4ac2-8157-ea297b137d55.png)](https://hubspot-cta-redirect-eu1-prod.s3.amazonaws.com/cta/redirect/25665555/13141798-b490-4ac2-8157-ea297b137d55) ##### References \[1\] Greiner Bio-One Microplate Selection Guide https://www.gbo.com/fileadmin/media/GBO-International/01\_Downloads\_BioScience/SALES\_Brochures/English/F073048\_microtiter plate \_Selection\_Guide\_EN.pdf ### Don't miss our regular updates on scientific topics around HTS Tags: [MICROPLATE SELECTION](https://focus.gbo.com/blog/hts/tag/microplate-selection) [Back to Blog](https://focus.gbo.com/blog/hts) - [Tweet](https://twitter.com/share) ## Related Articles ## [Choosing the Optimal Microtiter Plate for Best Results](https://focus.gbo.com/blog/hts/choosing-the-optimal-microtiter-plate-for-best-results) The gold standard in high-throughput microscopy is still glass microtiter plates (e.g., Greiner... [Read More](https://focus.gbo.com/blog/hts/choosing-the-optimal-microtiter-plate-for-best-results) ## [Essential Factors Influencing the Quality of HTS and Imaging](https://focus.gbo.com/blog/hts/essential-factors-influencing-the-quality-of-hts-and-imaging) The selection of a microtiter plate is often an iterative process that must balance competing... [Read More](https://focus.gbo.com/blog/hts/essential-factors-influencing-the-quality-of-hts-and-imaging) ## [Pushing boundaries in thermal shift assays towards 1536 wells](https://focus.gbo.com/blog/hts/pushing-boundaries-in-thermal-shift-assays-towards-1536-wells) Challenges of Drug Discovery - High attrition rates and costs risk Today, the vast majority of drug... [Read More](https://focus.gbo.com/blog/hts/pushing-boundaries-in-thermal-shift-assays-towards-1536-wells) FOOTER KOPIE - LEGAL - [Imprint](https://www.gbo.com/en-int/imprint) - [Data privacy](https://www.gbo.com/en-int/data-privacy) - [Terms & Conditions](https://www.gbo.com/en-int/terms-conditions) - [Compliance & Whistleblowing](https://www.greiner.com/en/greiner-group/compliance-guidelines/compliance/) - - - - © 2023 Greiner Bio-One International GmbH ```json { "@context" : "https://schema.org", "@type" : "BlogPosting", "author" : { "@type" : "Person", "name" : "Akin Töpfer", "url" : "https://focus.gbo.com/blog/hts/author/akin-töpfer" }, "dateModified" : "2023-10-31T10:21:21.640Z", "datePublished" : "2023-06-23T10:41:49.000Z", "headline" : "Optimizing HTS Microtiter Plate Selection with our Product Finder", "image" : [ "https://focus.gbo.com/hubfs/HTS%20Kampagne/HTS%20Microplate/CP01_Microplate%20Selection_Awareness_01_840x540px.jpg" ], "mainEntityOfPage" : { "@id" : "https://focus.gbo.com/blog/hts/optimizing-hts-microtiter-plate-selection-with-our-product-finder", "@type" : "WebPage" }, "publisher" : { "@type" : "Organization", "logo" : { "@type" : "ImageObject" }, "name" : "Greiner Bio-One International GmbH" } } ```